credibility of chromomycin a3 staining in prediction of fertility

نویسندگان

mohammad hossein nasr-esfahani

roshanak aboutorabi

shahnaz razavi

چکیده

background: chromomycin a3 (cma3) staining has been used to assess protamine deficiency. the aim of this study was to determine credibility of cma3 along with semen parameters for assessment of fertility potential. materials and methods: semen analysis and cma3 staining were carried out on 234 fertile and 178 subfertile individuals. semen analysis was assessed according to who criteria. protamine deficiency was assessed by cma3 staining. results: means, range of variables, coefficients of correlation and receiver operating characteristic (roc) analyses of semen parameters and protamine deficiency were determined. mean values of three main sperm parameters and the percentage of sperm with negative cma3 were significantly different between fertile and sub fertile groups. the results of cma3 assessment showed significant correlation with sperm density, percentage of motility and normal morphology in the total population, while in the subfertile group the results of cma3 showed significant correlation with sperm density and normal morphology. however in fertile men, the only significant correlation was observed between sperm with negative cma3 and normal morphology. roc analyses revealed that cma3 staining has a higher potential to predict fertility status, compared to semen parameters. conclusion: assessment of protamine deficiency could be considered as one of the complementary tests along with semen analysis for assessment of fertility.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Credibility of Chromomycin A3 Staining in Prediction of Fertility

Background Chromomycin A3 (CMA3) staining has been used to assess protamine deficiency. The aim of this study was to determine credibility of CMA3 along with semen parameters for assessment of fertility potential. MaterialsAndMethods Semen analysis and CMA3 staining were carried out on 234 fertile and 178 subfertile individuals. Semen analysis was assessed according to WHO criteria. Protamine d...

متن کامل

Chromomycin A3 Staining Indicates Sperm Protamine Deficiency

Chromomycin A3 binds to DNA and Protamine -deficient spermatozoa look bright yellow,whereas normal spermatozoa appear dull yellow.

متن کامل

Evaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining

Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...

متن کامل

prediction of ignition delay period in d.i diesel engines

a semi-empirical mathematical model for predicting physical part of ignition delay period in the combustion of direct - injection diesel engines with swirl is developed . this model based on a single droplet evaporation model . the governing equations , namely , equations of droplet motion , heat and mass transfer were solved simultaneously using a rung-kutta step by step unmerical method . the...

evaluation of protamine level in human sperm samples using chromomycin a3 and aniline blue staining

background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...

متن کامل

Biogenesis of chromomycin A3 by Streptomyces griseus.

The biosynthesis of chromomycin A3 was investigated using 13C-labeled acetates, methionine and glucose, and 13C,18O-labeled acetate. 13C NMR spectral analysis demonstrated that: Aglycone assembly occurs by combining at least two polyketide chains; three of nine oxygen atoms of the aglycone originate from acetate precursor oxygen atoms; carbon methylations on the aromatic ring at C-7, on the chr...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of fertility and sterility

جلد ۳، شماره ۱، صفحات ۵-۱۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023